OPTIMIZATION TRENDS IN TOTAL LIPOPEPTIDE PRODUCTION BY Bacillus Velezensis 0G REVEALED A SUSTAINABLE SUBMERGED FERMENTATION METHOD USING SWEET POTATO PEELS

Bacillus velezensis are plant-growth promoting microorganisms. They produce an interesting class of cyclic peptides; surfactin, which have antifungal and surfactant properties. Lipopeptide of the surfactin family are very potent biosurfactants with important applications for environmental remediation and chemical industries. In the present work, the complex production of lipopeptide (mostly surfactins) by B. velezensis 0G, was evaluated under different growth conditions using factorial design 23 with central and axial points. All experiments were carried out based on sustainable submerged fermentation containing sweet potato peels. The response surface trends demonstrate a maximum biosurfactant production at inoculum volume of 32 mL; combined broth volume of 46 mL, and incubation time of 24h. These observations were based on the analysis of supernatant by HPLC.