Influence of Nicotine Dependence, Current Smoking Status and Gender on CYP1A1, CYP1B1 and CYP2A6 Expression in Oral Squamous Cell Carcinoma

Objective: Tobacco smoke is composed of cancer-causing chemicals referred to as carcinogens. These carcinogensare metabolized by the enzymes of the cytochrome P450 (CYP) family. Our objective was to evaluate thecorrelation of tobacco consumption parameters with CYP1A1, CYP1B1 and CYP2A6 expression using qRT-PCRin samples of oral squamous cell carcinoma (OSCC). Material and Methods: The sample was divided into2 groups: Cancer (36 subjects) and non-Cancer (12 subjects). The smokers’ participants (36) were evaluatedregarding their Nicotine dependence (ND) was assessed by the Fagerström test for cigarette dependence (FTCD).Questions regarding tobacco consumption like the number of cigarettes/day (CPD), duration of use, and packyearswere also evaluated. The Mann-Whitney and Spearman correlation tests were used at a significance levelof 5%. Results: 48 participants were included, 32 men (66.7%), 36 smokers (75%) and 27 smokers with OSCC(56.3%). Samples of OSCC expressed more CYP1A1, CYP1B1, and CYP2A6. Especially, the CYP1B1 gene wassignificantly expressed in OSCC samples, regardless gender or tobacco use. No women expressed CYP2A6, aswell as, non-smokers did not express the CYP1A1 and CYP2A6 genes. CYP1A1 gene was higher among men(P = 0.021). Conclusion: Lack of exposure to tobacco may justify the absence of CYP1A1 and CYP2A6 expressionin non-smokers. The CYP1B1 gene was significantly expressed in the cancer presence despite gender or tobaccouse. The assessment of ND and quantification of tobacco consumption are important instruments in monitoringsmokers with benign oral lesions and, especially, in the presence of cancer.KEYWORDSCYP1A1; CYP1B1; CYP2A6; Carcinoma; Squamous cell; Tobacco use disorder.