Immobilization, Biochemical, Thermodynamic, and Fruit Juice Clarification Properties of Lignocellulosic Biomass–derived Exo-Polygalacturonase from Penicillium Paxilli
Biomass conversion and biorefinery（2022）
Abstract
Exo-polygalacturonase was produced from a fungal strain Penicillium paxilli under solid-state fermentation (SSF) technique using wheat and rice bran (1:1) as a substrate. Production optimization data suggested that yeast extract, pectin, and manganese sulfate significantly enhanced the exo-polygalacturonase yield. This enzyme was partially purified using NH4(SO4)2 precipitation at 70
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Key words
Lignocellulosic biomass,Exo-polygalacturonase,Kinetic constants,Thermal stability,Immobilization,Fruit juice clarification,Reusability
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