Generation and Application of TGFβ-educated Human Vγ9Vδ2 T Cells

Clinical trials that tested the antitumor activity of gd T cells have been mostly un-successful. To address this, we expanded human Vg9Vd2 T cells in TGFb1, a cyto-kine which enhances their viability, trafficking properties, and intrinsic antitumor activity. This protocol summarizes the production and in vitro functional charac-terization of TGFb1 educated human Vg9Vd2 cells and highlights their compati-bility with chimeric antigen receptor (CAR) engineering. We also describe in vivo testing of the antitumor activity of these CAR T cells in mice.For complete details on the use and execution of this protocol, please refer to Beatson et al. (2021).