Targeted long-read sequencing reveals clonally expanded HBV-associated chromosomal translocations in patients with chronic hepatitis B

•Fresh frozen liver biopsies from patients with CHB were subjected to targeted long-read RNA and DNA sequencing.•Inter-chromosomal translocations associated with HBV integration events detected in one-third of patients.•Chromosomal translocations were unique to each biopsy sample, suggesting that each originated randomly.•A larger fraction of the HBV transcriptome originates from cccDNA in patients who are HBeAg-positive.