Evaluation of N6-adenine DNA-immunoprecipitation-based Genomic Profiling in Eukaryotes

The detection of low-abundance DNA N6-methyladenine (DNA-m6A) remains challenging, limiting our understanding of this novel base in eukaryotes. To address this, we introduce an approach for systematically validating the selectivity and sensitivity of antibody-based DNA-m6A methods, revealing most commercial antibodies as poorly selective towards DNA-m6A. Finally, using a validated highly selective anti-DNA-m6A antibody we expose distinct patterns of DNA-m6A in C. reinhardtii , A. thaliana , and D. melanogaster . ### Competing Interest Statement A.B.S. is a coinventor on a U.S. patent application that includes discoveries described in this manuscript.