Stable Chlorine Dioxide Combined with Slightly Acidic Electrolyzed Water: an Effective Way to Delay Protein Deterioration of Refrigerated Large Yellow Croaker (pseudosciaena Crocea)

Summary Fish is susceptible to spoilage for enzymatic and microbial activities during storage, proteolysis of MPs by endogenous proteases played a crucial influence in the early postmortem decomposition of myosin. This study aimed to investigate the impact of stable chlorine dioxide (ClO 2 ) combined with slightly acidic electrolyzed water (SAEW) on myofibrillar proteins (MPs) and endogenous proteases alterations in large yellow croaker ( Pseudosciaena crocea ) during cold storage. The results of Fourier‐transform infrared spectroscopy (FT‐IR) and intrinsic fluorescence intensity (IFI) showed that the ClO 2 + SAEW treatment kept the structural stability of protein in fillets during cold storage. Moreover, ClO 2 + SAEW treatment dramatically inhibited protein degradation and oxidation. The results of light microscopy further confirmed the results. Meanwhile, there was a tendency to first increase and then decrease on the activities of calpain and cathepsin B, L. According to the correlation analysis and principal component analysis, the activities of endogenous proteases (cathepsin B, D, L, and calpain) were significant ( P < 0.01) positively correlated to myofibrillar fragmentation index and trichloroacetic acid‐soluble peptide, and were significant ( P < 0.01) negative correlated with the contents of total sulfhydryl and Ca 2+ ‐ATPase. The endogenous proteases activities and protein deterioration were strongly associated. Therefore, endogenous protease was affected by ClO 2 and SAEW, which delayed protein degradation in large yellow croaker, especially ClO 2 combined with SAEW treatment.