Effect of Intestinal Bubble Accumulation on Antioxidant Enzyme and Lysozyme Activities in the Tissues of Pampus Argenteus

In order to investigate the effect of intestinal bubble accumulation on the antioxidant capacity of silver pomfret Pampus argenteus, fifteen diseased fish were chosen as diseased group[(11.7±0.8)cm, (42.8±4.1)g] and fifteen healthy fish as control group [(12.5 ± 0.7)cm, (48.6 ± 5.6)g]. These fish were collected from Fuding research center of East China Sea Fisheries Research Institute in March 2019. Liver, kidney, muscle, and gill tissues of each group were collected and used to test their superoxide dismutase activity, catalase activity, malondiadehyde content and total antioxidant capacity, and kidney and gill tissues of each group were collected and used to test their lysozyme activity. The experimental results showed that the total antioxidant capacity of liver, gill and kidney of the diseased fish group decreased, and it decreased significantly in the liver and kidney(P <0.05), and the total antioxidant capacity in muscle was significantly higher than that in the control group (P<0.05); the activity of superoxide dismutase and catalase in liver and muscle of the diseased group was significantly lower than that in the control group (P<0.05); the malondiadehyde content in liver and muscle was significantly higher than that in the control group (P<0.05), but the malondiadehyde content in gill and kidney were significantly lower than those in the control group (P<0.05); lysozyme activity of gill was significantly higher than that in the control group (P<0.05). Results show that: 1) During the intestinal bubble accumulation, the antioxidant enzyme activity of liver and muscle tissues of Pampus argenteus is impacted strongly. The antioxidant enzyme activity indicators of these two tissues can be used for the detection and pathological analysis of the disease; 2) The accumulation of bubbles in fish intestines is likely to be accompanied by bacterial infections, but still needs further verification.