Effect of different laser wavelength on callus of date palm.

The present work was carried out to investigate the effects of red diode laser at wavelength 650 nm power 7 mw and green diode laser at wavelength 532 nm power 160 mw on palm callus culture. The callus were exposed to red laser for 5, 10, 15 and 20 minutes and 4,8,12 and 16 minutes to green laser. In most cases the highest content of total soluble amino acids and total phenols were obtained from the treatment of 20 min in red laser and treatment of 16 min in green laser. The highest values of Total soluble sugars, Total Indol were obtained from the treatment of 10 min in red laser and treatment of 8 min in green laser. Electrophoreses protein and DNA analysis was carried out to study the genetic similarity between different time of Laser irradiation with palm callus cultures. DNA analysis which performed a great similarity between control and 10 min treatment reached to 92% while Lower similarity between control and 15 min treatment reached to 84%