Determination of the Optical Properties in Normal and Diseased Tissues by Novel Goniometry and by 3D Second Harmonic Generation Microscopy (conference Presentation)

We present two new methods used to determine the optical properties in ovarian cancer, idiopathic pulmonary fibrosis and osteoarthritis. The bulk optical properties are determined by a combination of on axis attenuation, goniometry, and Monte Carlo simulations. The resulting wavelength dependent values delineates the importance of both μs’ and the fractal dimension. We also present a new method to determine μs’ on the SHG microscope. By measuring the depth dependence of the SHG emission directionality and performing Monte Carlo simulations, both the relative fibril size distribution and reduced scattering coefficient can be simultaneously determined without the need for bulk measurements.