Bioinformatic pipeline for studying transcriptome and regulome dynamics during neural differentiation v5
protocols.io(2019)
摘要
Here we present a bioinformatic pipeline for dissecting transcriptional regulation of neural differentiation process. Single cell RNA-seq and bulk ATAC-seq with two biological replicates was applied to the indicated cell stages including human induced pluripotent stem cells (hiPSCs), embryoid body (EB), early rosettes (Ros-E), late rosettes (Ros-L), neural progenitor cells (NPCs), and the original somatic fibroblasts (Fib) for a deeper understanding of the regulatory mechanisms driving the differentiation of the neural lineage. This pipeline could be applied to study transcriptome and regulome dynamics of other lineages.
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