In Vitro Evaluation of Mycobacterial Activity of Phytomedicine Mathesia on Mycobacterium Tuberculosis
Medical & clinical reviews(2018)
摘要
Introduction: At the international level, Directly Observed Treatment Short-Course (DOTS) is a public health strategy to effectively fight Tuberculosis (TB) epidemic. However, because of the irrational use of anti-tuberculosis drugs, Mycobacterium tuberculosis is developing more and more resistance to antibiotics and the number of cases of multidrug-resistant Tuberculosis (MR-TB and XDR-TB) is increasing. Thus, the therapeutic arsenal used in this context becomes limited and the care also becomes difficult. Objective: The study aims to demonstrate in vitro efficacy of MATHESIA on Mycobacterium tuberculosis. Methods: Thirty eight (38) positive Ziehl or Auramine sputum from failures and relapses cases were collected. After treatment with 4% sodium hydroxide, the direct method of proportion technique was used. It consisted of inoculating the pellets on Lowenstein-Jensen's medium (LJ), on incorporated LJ of MATHESIA at different concentrations and on LJ incorporated anti-tuberculosis drugs with well-known critical concentrations. The tubes were incubated at 37°C and read at 28 and 42 days for susceptibility testing. Results: Six strains (15.8%) were sensitive to all the anti- TB drugs tested and also to MATHESIA. In contrast, 32 strains (84.2%) were resistant to at least one antituberculosis drug. In contrast to MATHESIA, all the 32 strains resistant to at least one antituberculous drug showed growth only on a single concentration (0.01 mg/ml) whereas in the other 4 concentrations, growths were not observed, even for 21 MDR-TB souches (55.3%). Conclusion: The anti-mycobacterial activity is certain in the MATHESIA and more in-depth research must be done to determine its behavior in the treatment.
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