ASSESSMENT OF STANDARDIZED EXTRACT OF PROPOLIS (EPP-AF ®) ON IMMUNE RESPONSE IN EXPERIMENTAL CANDIDEMIA.

Event Abstract Back to Event ASSESSMENT OF STANDARDIZED EXTRACT OF PROPOLIS (EPP-AF ®) ON IMMUNE RESPONSE IN EXPERIMENTAL CANDIDEMIA. Thiare S. FORTES1*, Mayara Cristina P. Da Silva1, Marcia C. Maciel1, Diego S. Arruda1, Ana P. Dos Santos2, Andresa A. Berreta3 and Flávia R. Nascimento4 1 UFMA, Center for Biological and Health Sci ence, Brazil 2 UFMA, Physyology, Brazil 3 Apis Flora Indl. Coml. Ltd, Development & Innovation, Brazil 4 UFMA, Pathology, Brazil Introduction. Propolis is a resinous balsamic material with a complex chemical composition. It is produced by bees from material collected from flowers with addition of mandibular secretions. This material is used for the protection of the hive front of infectious agents such as fungi, bacteria, viruses and insects. Among the antimicrobial activities of propolis have proven, the antifungal action against strains of Candida albicans. Aim: Evaluate the effect of standardized extract of propolis (EPP-AF ®) in immunosuppressed mice and infected by c. albicans. Mice of the C57Bl/6 strain were immunosuppressed for a week with dexamethasone (3 mg/kg) and in the eighth day were infected with c. albicans yeasts (2x106) via intraperitoneal. From the ninth day, the animals were separated into 3 groups. The control group received PBS and the other two groups received EPP-AF ® in doses of 10 (EPP-AF ® 10) and 100 mg/kg (EPP-AF ® 100). All treatments were intraperitoneal. After 7 days of treatment, half the animals were sacrificed to assess the number of cells linfoides organs (bone marrow, spleen and lymph nodes mesenteric) and peritoneal washing. For analysis of survival, the other half of the animals continued to be treated for over 8 days and were evaluated until the 20th day post-infection sweat. Results: in relation to survival, 100% of the animals of the group EPP-AF ® 100 remained alive even after 20 days of infection. This survival was higher when compared to Control groups and EPP-AF ® 10. In relation to organ linfoides cellularity, animals in the group EPP-AF ® 100 showed a significant increase in the number of bone marrow cells in relation to the control group and the EPP-AF ® 10. The increased production of bone marrow cells was accompanied by an increase in the number of cells in the peritoneal and spleen washed in the group, EPP-AF ® 100 when compared to the control group. However, this profile is not repeated in relation to the number of cells of the mesenteric lymph node, since the group EPP-AF ® 100 presented a significant reduction in the number of cells in the control group and the group EPP-AF ® 10. These results suggest that immune cells may be going through a process of activation, which reflects on the largest bone marrow cell production and increased cell proliferation in esplenócitos, at the same time, the reduction of cells in the lymph nodes can be recruited into the infectious focus. This picture, could justify the greater survival of animals. Conclusion: the EPP-AF ® acts as an immunostimulant of the response of the host immune suppressed, inducing production, proliferation and recruitment of immune cells activated and, consequently, increased survival post candidemia. Acknowledgements I thank the incentive programs FAPEMA, CAPES, CNPq and Apis Flora Indl. Coml. Ltda. They had great contribution to the development and completion of this paper. Keywords: Standardized propolis extract (EPP-AF ®), Immune Modulation, Experimental candidemia, immune response, microbiome and immune system Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Poster Presentation Topic: Mucosal Immunity and the microbiome Citation: FORTES TS, Da Silva MP, Maciel MC, Arruda DS, Dos Santos AP, Berreta AA and Nascimento FR (2015). ASSESSMENT OF STANDARDIZED EXTRACT OF PROPOLIS (EPP-AF ®) ON IMMUNE RESPONSE IN EXPERIMENTAL CANDIDEMIA.. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00245 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 24 Apr 2015; Published Online: 14 Sep 2015. * Correspondence: Miss. Thiare S FORTES, UFMA, Center for Biological and Health Sci ence, São Luís, Maranhao, 65085580, Brazil, thiarefortes@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Thiare S FORTES Mayara Cristina P Da Silva Marcia C Maciel Diego S Arruda Ana P Dos Santos Andresa A Berreta Flávia R Nascimento Google Thiare S FORTES Mayara Cristina P Da Silva Marcia C Maciel Diego S Arruda Ana P Dos Santos Andresa A Berreta Flávia R Nascimento Google Scholar Thiare S FORTES Mayara Cristina P Da Silva Marcia C Maciel Diego S Arruda Ana P Dos Santos Andresa A Berreta Flávia R Nascimento PubMed Thiare S FORTES Mayara Cristina P Da Silva Marcia C Maciel Diego S Arruda Ana P Dos Santos Andresa A Berreta Flávia R Nascimento Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. 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