Recombinant Escherichia coli Cell Lysates as a Low-Cost Alternative for Vaccines Against Veterinary Clostridial Diseases.

Clóvis Moreira,Gustavo Marçal Schmidt Garcia Moreira,Rafael Amaral Donassolo,Marcos Roberto Alves Ferreira,Mariliana Luiza Ferreira Alves,Rafael Rodrigues Rodrigues,Fabricio Rochedo Conceição

Methods in molecular biology (Clifton, N.J.)（2022）

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Abstract
This chapter describes a practical, industry-friendly, and efficient vaccine protocol based on the use of Escherichia coli cell fractions (inclusion bodies or cell lysate supernatant) containing the recombinant antigen. This approach was characterized and evaluated in laboratory and farm animals by the seroneutralization assay in mice, thereby showing to be an excellent alternative to induce a protective immune response against clostridial diseases.
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Key words
Cell lysate supernatant,Clostridiosis,Inclusion bodies,Recombinant antigens,Recombinant vaccines
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