High Levels of C-Reactive Protein Are Associated with Ischemic Stroke Short-term Outcome in Patients with the T Allele of the CRP Rs1130864 Variant

Objective: C-reactive protein (CRP) has been associated with Ischemic Stroke (IS) risk and short-term outcome. We evaluated the association between the CRP rs1130864 variant (+1444 C>T) with susceptibility and short-term outcome of IS and levels of CRP. Methods: We enrolled 168 IS patients and 166 controls. Baseline characteristics and blood samples were obtained up to 24 hours of hospital admission. The disability was evaluated using the Modified Rankin Scale (mRS) after three months and categorized as mild (mRS<3) and moderate/severe (mRS≥3). The rs1130864 genotyping was determined using polymerase chain reaction and restriction fragment length polymorphism. Serum levels of CRP were determined using high sensitivity turbidimetric assay (hsCRP). Results: Sex, hypertension, smoking and hsCRP levels were associated with IS. The median of hsCRP was 7.5 mg/L in IS patients and 1.6 mg/L in controls (p<0.001). The rs1130864 genotype distribution did not differ between the groups. However, controls carrying the T allele (CT+TT genotypes) showed higher hsCRP (p=0.005) and more frequency of hsCRP ≥3 mg/L than those carrying the CC genotype (p=0.045). Age and hsCRP predicted moderate/severe disability after three-month only in patients carrying the T allele (p<0.001). Daniela Frizon Alfieri1; Tamires Flauzino1; Francieli Delongui1; Márcio Francisco Lehmann2; Maria Caroline Martins De Araújo3; Rafaele Maria Tirolla4; Nicolas Pivoto5; Elaine Regina Delicato de Almeida1,6; Andréa Name Colado Simão1,6; Mariana Ragassi Urbano7; Edna Maria Vissoci Reiche1,6* 1Laboratory of Research in Applied Immunology, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil. 2Department of Clinical Surgery, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil. 3Outpatient Clinic for Neurology, University Hospital, State University of Londrina, Londrina, Paraná, Brazil. 4Pharmacy School, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil. 5Medicine School, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil. 6Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil. 7Department of Statistics, State University of Londrina, Londrina, Paraná, Brazil. keywords: Ischemic stroke; rs1130864; C-reactive protein; Short-term outcome; Disability.