Light Environment Effect in the Sperm and Ribonucleic Acid Quality and Body Weight of Male Mus musculus

BACKGROUND: The mice are categorized as the nocturnal animal meaning that most of the activity conducted in dark environment. However, the treatment of mice as the object studied in the laboratory often experiences the lack attention especially the treat to light exposure to mice during the investigation period which potentially affect the result. AIM: The present study aims to investigate the effect of light environment in the sperm quality, RNA quality, and body weight of male Mus musculus. METHODS: We compared in the mice the desired parameter onto the sample of 16 mice which kept in light and dark cages for 6 weeks. RESULTS: The results indicated that there was a significant difference in the number of sperm, concentration, and purity of RNA between the mice exposed in dark and light environment. The average number of sperm from the ones kept in the dark was found twice as much as those in the light condition. The average RNA concentration in the dark was higher than in the light place but the RNA purity in the dark was lower than the light place. However, the motility and morphology of sperm was not showing any significancy different in both conditions. CONCLUSION: The result proved that the natural light of cages for treating the mice in the laboratory influences the amount of sperm and the quality RNA. Therefore, the control of the light room in the experimental laboratory needs to be considered, especially during the further experiment which related to the quality of sperm, RNA, and body weight of mice.