Cervical Cutaneous Tuberculosis Shows the Inflammatory Infection Environment of Local Necrotic Granulation Tissue through the Expression Profile of Chemokine

Abstract Background Tuberculosis is an important infectious disease that jeopardizes human health. Research on extrapulmonary tuberculosis is still rare, especially cutaneous tuberculosis, a more refractory extrapulmonary tuberculosis. This study is to explore the inflammatory and immunobiomarkers of patients with cutaneous tuberculosis and to analyze the potential functions and pathways involved in the pathogenesis of M. tuberculosis (Mtb) infection, in order to support cutaneous tuberculosis diagnosis and treatment.Methods Analysis of mRNA identifies biomarkers in cutaneous tuberculosis and non-specific infectious ulcer tissue. Analyze the correlation between gene ontology (GO) and the Kyoto Gene and Genomic Encyclopedia (KEGG) biological pathways, immunity, inflammation and chemokines. Chemotaxis factors such as CXCL9, CXCL10, CXCL11 and CXCR3 were compared by quantitative PCR analysis.Results The mRNA expression levels of CXCL9, CXCL10, CXCL11 and CXCR3 in local necrotic granulation tissue of cutaneous tuberculosis were significantly increased compared with those of normal tissues. GO analysis indicated that activation of various immune responses and inflammatory responses may be associated with up-regulation of chemokine genes. Gene Ontology (GO) and Kyoto Biogenomics and Genomics Encyclopedia (KEGG) biological pathway analysis revealed the relationship between immune, inflammatory and chemokine pathways.Conclusion GO analysis and KEGG analysis of differentially expressed mRNA revealed potential functions and pathways associated with the onset of Mtb infection.