Application of the Scanning Ion-Conductance Microscopy (SICM) in Study of Voriconazole Impact on Candida Parapsilosis Surface Structure.

The studying of antifungal drugs effect on the surface structure by traditional microbiological methods is unobtainable. SICM method allows to obtain the topography of the surface structure and its mechanical properties of biological samples in non-contact mode (Clarke et al., 2016). Achievable to obtain a data of cells stiffness by applying pressure between of the nanopipette tip a sample surface. Azole drugs inhibit the synthesis of ergosterol (a component of the yeast membrane) (Ghannoum & Rice, 1999), which lead to destruction and softening of candida cell membrane. There are reports of the antifungal effect of caspofungin on elasticity of cell wall (Quilès et al., 2017); impact of azole drugs on Candida surface structure (Madhavan et al.,