Characterization of Potential Enzyme-Producing Bacteria from Kitchen Waste

Bacillus subtilis is non pathogenic gram positive bacteria that has the potential to produce numerous organic substances of industrial importance. The present research work was focused on the isolation and partial characterization of soil bacteria that can produce amylase, cellulase, lipase, and proteases. This bacterial ability could further be utilized for the effective degradation of food waste generated from households and hotels. We find the bacterial isolate viz., KWB 4 exhibited alpha amylase, beta amylase, protease, cellulase, and lipase activity. KWB 4 was identified as Bacillus sp. that exhibited 97% similarity to Bacillus subtilis as per the morphological and biochemical characteristics. Further, several optimizations and activity enhancement approaches could be applied to B. subtilis (KWB 4) to ensure the effective degradation of food wastes.