The Effects Of Acetamide On N-Acetyl-Beta-D-Glucosaminidase (Nagase) Activity And Sperm Motility In Semen From Boar

This research aimed to understand the effects of acetamide upon N-Acetyl-beta-D-glucosaminidase (EC 3.2.1.52, NAGase) activity in semen and sperm from the boar and to investigate the relationship of NAGase activity with sperm motility and fertilization rate. NAGase activity from semen of the boar was determined using the kinetic method of substrate reaction to study the enzyme catalyzed hydrolysis of p-nitrophenyl-N-acetyl-beta-D-glucosaminide. Different concentrations of acetamide were added to fresh semen and then incubated for different time periods at 37 degrees C. Hamilton Thorne computer-assisted sperm analysis was used to test various parameters that reflect sperm motility. Results showed that acetamide dose-dependently inhibited NAGase activity (IC50 =15 mmol/L), and provided reversible noncompetitive inhibition with an inhibition constant of 2.23 x 10(-2) mmol/L. The addition of 15-200 mmol/L acetamide to semen significantly decreased the percentage of progressive motile sperm, and the curvilinear, straight line and average path velocity, and lateral head displacement of sperm after incubation for 15-60 min (P < 0.01). Sperm motility was significantly decreased after 60 min of incubation (P < 0.05). These data suggest that acetamide had a strong inhibitory effect on semen NAGase, and could significantly affect sperm motility parameters. (C) 2019 Friends Science Publishers