Callusogenesis Of Eggplant (Solanum Melongena L.)

The eggplant (Solanum melongena L.) provides a unique system to study morphogenesis. The aim of the current work was to investigate the effect of some phytohormones and their combinations on callus induction in eggplant tissue cultures. Two eggplant cultivars, Larga Negra and Black Beauty were chosen for the study. Cotyledons and hypocotyls from 30-day-old seedling were the best explants for callus induction. Murashige and Skoog (MS) medium supplemented with naphthalene acetic acid (NAA)-2.0 mg/l and 6-benzylaminopurine (BAP)-0.5 mg/l proved to be very suitable for callus induction. Callus was obtained from 90.0% of cotyledon explants and from 63.3% of hypocotyl explants from cultivar Larga Negra. All kinds of calli were used to produce organogenesis.