Brucella Phage Lysate Bacterin Induces Elevated Tlrs And Cytokines Response In Murine Model

TLRs sense pathogen associated molecular patterns of invading microbes which initiate intracellular signaling cascades and lead to production of pro-inflammatory cytokines which in turn help in bridging innate and adaptive immune response. TLRs and cytokines response of plain and alum adsorbed lysate of Brucella abortus strain 19 in mice model were evaluated by relative expression of the transcript by real time PCR. Three different groups of mice immunized with plain lysate bacterin, alum adjuvanated lysate, standard B. abortus strain 19 vaccine respectively and an unimmunized control group were used in present study. The results showed higher expression of TLR2, TLR4, TLR9, IL-4, IL-12 and INF-alpha transcripts in spleen of all the immunized mice groups as compared to unimmunized control groups. The relative higher transcripts expression of TLRs and cytokines in alum adjuvanated lysate as comparison to plain lysate bacterin suggest better immuno-protective response of alum adjuvanated phage lysate bacterin against the brucellosis.