The Molecular Responses Of Human Embryonic Kidney Cells To Abnormal Expression Of Rna Binding Motif Protein 10: Cues To Molecular Mechanism And Function

This study was aimed to illustrate the role of RNA binding motif protein 10 (RBM10) in the human embryonic kidney (HEK) 293 cells. The RNA sequencing data following RBM10 knockdown (KD) or over-expression (OE) in HEK293 cells were downloaded and reanalyzed. Differentially expressed genes (DEGs) were screened and divided into four groups, including RBM10 KD induced up-and down-regulated, RBM10 OE induced up-and down-regulated groups. In addition, direct (differentially expressed in RBM10 OE and KD groups) and indirect DEGs (differentially expressed in one groups) were identified, and protein-protein interaction (PPI) network for them was constructed. In the end, pathway enrichment analysis of genes was performed. Total 268 DEGs (169 up-and 99 down-regulated) in RBM10 KD group and 113 DEGs (38 up and 75 down-regulated) in RBM10 OE group were obtained, of which, 34 DEGs were direct genes. A direct-indirect PPI network with 339 nodes including 18 direct DEGs, 156 indirect DEGs and 165 intermediate genes was gained. SAT1 (spermidine/spermine N1-acetyltransferase 1), HMBOX1 (homeobox containing 1), CLOCK (clock circadian regulator) and CCND2 (cyclin D2) with high degree were direct genes. Genes in PPI network were closely associated with signaling pathways related to cell cycle and cancers. In conclusion, RBM10 may be involved in the regulation of gene expression of CLOCK, SAT1 and CCND2. RBM10 may interact with these genes to involve in gene expression and congenital disease occurrence through multiple signaling pathways including dopaminergic synapse and Wnt and Hippo signaling pathway.