Mirna-145 And Igf-1r In Hydrosalpinx-Induced Infertility

Objective: To explore the expression of miRNA-145 and insulin-like growth factor 1 receptor (IGF-1R) in endometrial tissues of patients with hydrosalpinx-induced infertility and their influencing mechanism. Methods: A total of 37 patients with hydrosalpinx infertility (hydrosalpinx group) who visited our hospital from January to September 2019 were retrospectively analyzed. In the same period, 30 patients with endometriosis (EMs) and 28 normal women underwent abortion operation were selected as the EMs group and the control group, respectively. Meanwhile, patients in the hydrosalpinx group were divided into mild group, moderate group and severe group based on the degree of hydrosalpinx. Endometrial tissues were obtained from all study subjects. Real-time fluorescence quantitative PCR was used to determine the expression level of miRNA-145 in endometrial tissues, and IGF-1R expression level was measured by enzyme-linked immunosorbent assay. The expression levels of miRNA-145 and IGF-1R in endometrial tissues of the three groups of study subjects and patients with different degrees of hydrosalpinx were compared, and their correlations with hydrosalpinx were analyzed. Results: The age, parity and the number of cesarean sections in the hydrosalpinx group and EMs group were significantly higher than those in the control group (all P < 0.05), while there was no significant difference between hydrosalpinx group and EMs group (all P > 0.05). The expression level of miRNA-145 in the endometrial tissues of the hydrosalpinx group was significantly higher than that in the EMs group and control group (all P < 0.001), while the expression level of IGF-1R was significantly lower than that in the EMs group and control group (all P < 0.001). With the aggravation of hydrosalpinx, miRNA-145 expression showed an increasing trend and IGF-1R showed a decreasing trend in endometrial tissues of hydrosalpinx patients, and there were significant differences among the three groups (all P < 0.001). Pearson correlation analysis showed that there was a significant negative correlation between miRNA-145 expression level and IGF-1R in endometrial tissues of patients with hydrosalpinx infertility (r = -0.652, P < 0.01), and multiple linear regression analysis showed that there was a significant correlation between the severity of hydrosalpinx and miRNA-145 and IGF-1R expression levels (P < 0.001). Conclusion: The abnormal expression of miRNA-145 and IGF-1R may be an important mechanism affecting infertility caused by hydrosalpinx.