Study On The Related Cracking-Resistant Genes In Chinese Jujube

The problem of fruit cracking for Chinese jujube (Zizyphus jujuba Mill.) causes serious yield losses in China, however, the existing prevention and control measures are hard to solve it. Two groups of high resistant cracking type and easy cracking type for the filial generation of Dong Zao x Linyili Zao were divided in the study. Two allelic genetic pool and the related genes cDNA libraries for the cracking-resistant characters to be specifically expressed were built to conduct the preliminary feasibility study on the genes related to the resistance of fruit-cracking through the differently expressed genes. The sequencing production of the two group transcriptomes were received more than 4.5 Gb and 4.6 Gb data after removing the low quality segments, and conducted 45,401,606 and 46,468,222 times reading, the Q20 (the base ratio no less than 20) of the two libraries were both greater than 96%. After comparing the genetic expression within two groups, 391 items of differently expressed genes (DEGs) were filtered. Genome function annotation on the 391 items DEGs were conducted and the results showed that there were 92 items genes added, 45 items defined, and 299 items gene without annotation. The annotated genes probably involving in jujube cracking phenomenon were Aquaporin PIP, Tubulin, Calreticulin and Calmodulin.