Chrome Extension
WeChat Mini Program
Use on ChatGLM
VIPCreated with Sketch.

    • Cell Viability Assessment Using Fluorescence Vital Dyes and Confocal Microscopy in Evaluating Freezing and Thawing Protocols Used in Cryopreservation of Allogeneic Venous Grafts

    Pavel Mericka,Libor Janousek,Ales Benda, Radka Lainkova,Jan Sabo,Marketa Dalecka, Petra Proksova,Myroslav Salmay,Rudolf Spunda,Ondrej Pecha,Miroslava Jandova,Jiri Gregor,Lubomir Sterba,Miroslav Spacek,Jaroslav Lindner

    International journal of molecular sciences（2021）SCI 2区SCI 3区

    Univ Hosp | Inst Clin & Expt Med | Charles Univ Prague | Czech Acad Sci

    Cited 5|Views43
    Abstract
    The authors present their contribution to the improvement of methods suitable for the detection of the freezing and thawing damage of cells of cryopreserved venous grafts used for lower limb revascularization procedures. They studied the post-thaw viability of cells of the wall of cryopreserved venous grafts (CVG) immediately after thawing and after 24 and 48 h culture at +37 °C in two groups of six CVG selected randomly for slow thawing in the refrigerator and rapid thawing in a water bath at +37 °C. The grafts were collected from multi-organ and tissue brain-dead donors, cryopreserved, and stored in a liquid nitrogen vapor phase for five years. The viability was assessed from tissue slices obtained by perpendicular and longitudinal cuts of the thawed graft samples using in situ staining with fluorescence vital dyes. The mean and median immediate post-thaw viability values above 70% were found in using both thawing protocols and both types of cutting. The statistically significant decline in viability after the 48-h culture was observed only when using the slow thawing protocol and perpendicular cutting. The possible explanation might be the “solution effect damage” during slow thawing, which caused a gentle reduction in the graft cellularity. The possible influence of this phenomenon on the immunogenicity of CVG should be the subject of further investigations.
    More
    Translated text
    Key words
    cell viability,vascular allograft,fluorescence vital dyes,confocal microscopy,cryopreservation,thawing method
    求助PDF
    上传PDF
    View via Publisher
    Bibtex
    AI Read Science
    AI Summary
    AI Summary is the key point extracted automatically understanding the full text of the paper, including the background, methods, results, conclusions, icons and other key content, so that you can get the outline of the paper at a glance.
    Example
    Background
    Key content
    Introduction
    Methods
    Results
    Related work
    Fund
    Key content
    • Pretraining has recently greatly promoted the development of natural language processing (NLP)
    • We show that M6 outperforms the baselines in multimodal downstream tasks, and the large M6 with 10 parameters can reach a better performance
    • We propose a method called M6 that is able to process information of multiple modalities and perform both single-modal and cross-modal understanding and generation
    • The model is scaled to large model with 10 billion parameters with sophisticated deployment, and the 10 -parameter M6-large is the largest pretrained model in Chinese
    • Experimental results show that our proposed M6 outperforms the baseline in a number of downstream tasks concerning both single modality and multiple modalities We will continue the pretraining of extremely large models by increasing data to explore the limit of its performance
    Upload PDF to Generate Summary
    Must-Reading Tree
    Example
    Generate MRT to find the research sequence of this paper
    Data Disclaimer
    The page data are from open Internet sources, cooperative publishers and automatic analysis results through AI technology. We do not make any commitments and guarantees for the validity, accuracy, correctness, reliability, completeness and timeliness of the page data. If you have any questions, please contact us by email: report@aminer.cn
    Chat Paper
    【要点】：本文研究了使用荧光活细胞染料和共聚焦显微镜评估冷冻保存的同种异体静脉移植物在慢速和快速解冻后的细胞存活率，以改进冷冻保存方法的检测手段。
    

    【方法】：作者采用荧光活细胞染料原位染色，对解冻后的静脉移植物壁组织切片进行细胞活力评估。
    

    【实验】：实验选取了两组各六份随机选取的冷冻保存静脉移植物，分别采用冰箱内慢速解冻和水浴中快速解冻两种方法，并在解冻后立即、以及37°C培养24小时和48小时后进行细胞活力评估。移植物来源于多器官和组织脑死亡捐献者，经冷冻保存并在液氮气相中存储五年。结果显示，两种解冻方法下，细胞活力均值和中位数在70%以上，仅在慢速解冻和垂直切割的样本中，48小时培养后观察到细胞活力显著下降。