Antiviral Iridoid Glycosides from Clerodendrum Myricoides

The methanol root extract of Clerodendrum myricoides (Hochst.) Vatke afforded two new (1, 2) and two known (3, 4) iridoid glycosides. The structures of the isolated compounds were established based on NMR, IR, UV and MS data analyses. The crude extract and the isolated constituents were assayed for antiviral activity against the human respiratory syncytial virus (RSV) in human laryngeal epidermoid carcinoma (HEp-2) cells. The crude extract inhibited RSV infectivity at EC50 = 0.21 mu g/ml, while it showed cytotoxicity against HEp-2 cells with CC50 = 9 mu g/ml. Compound 2 showed 43.2% virus inhibition at 100 mu M, while compounds 1 as well as 3 and 4 had only weak antiviral and cytotoxic activities.