Phytochemical Analysis And Antioxidant Activity Of N-Hexane And Ethyl Acetate In Flower Sphagneticola Trilobata (L.) J.F Pruski

The S. trilobata flowers grow thro ughout the year, so they are rarely used and eventually wasted. S. trilobata flowers contain metabolite compounds that have potential as antioxidants. Antioxidants are useful for reducing free radical activity which can cause degenerative diseases. The purpose of the study was to determine the antioxidant activity of S. trilobata flower extract in n-hexane and ethyl acetate solvents and to measure the value of antioxidant activity of n-hexane extract and ethyl acetate extract of S. trilobata flowers expressed by IC50. Extraction of S. trilobata flowers used the maceration method and measurement their antioxidant activities were carried out by the DPPH (2,2-diphenyl-1picrylhydrazyl) method with a spectrophotometer UV-Vis at a wavelength of 517. The results exhibited that the n-hexane extract had weak antioxidant activity with an IC50 value of 410.18 μg / mL, while the ethyl acetate extract had strong antioxidant activity with an IC50 value of 249.50 μg/mL.