ADIPOR1 regulates genes involved in milk fat metabolism in goat mammary epithelial cells
Research in veterinary science(2021)
摘要
Background: Fat metabolism is a complex process regulated by a number of factors. Adiponectin receptor 1 (ADIPOR1) gene takes active part in lipid metabolism. Although, there have been some researches indicating that ADIPOR1 could influence the milk fat metabolism through targeting some factors, little is known about the effect of ADIPOR1 on goat milk fat metabolism. To investigate the regulatory role of ADIPOR1 on milk fat metabolism in GMECs, we analysed overexpression in the presence and absence of AdipoRon (50 mu M) and examined knockdown using siRNA. Using RT-qPCR, we assessed ADIPOR1 mRNA expressions among different lactation stages in goat mammary gland and the expression of six genes that regulate milk fat metabolism in GMECs. Results: ADIPOR1 mRNA expression level was higher during the various lactation stages, except dry-off period. Knockdown and overexpression results revealed a significant decrease and increase in mRNA expression of ADIPOR1 and genes considered: SREBF1, ACACA, FASN, SCD, ATGL, and HSL, respectively. Treatment of GMECs with AdipoRon 50 mu M resulted in a significant (p < 0.05) increase in the mRNA expression of all measured genes, except SREBF1. Conclusion: Overall, ADIPOR1 plays a central role in regulating the transcription of several genes involved in milk fat metabolism.
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关键词
ADIPOR1,Milk fat metabolism,GMECs,AdipoRon
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