In-Silico Designing a Multi-Peptide Vaccine: Against Vibrio Cholera

Gram-negative bacilli are one of the main causes for development of cholera, causing intestinal infections and acute watery diarrhea. Vaccination is one of the most important ways to prevent such infections. Some important immunogenic factors, such as toxin-co-regulated pilus A (TcpA), outer membrane protein W (OmpW), and cholera toxin B subunit (ctxB) as adjuvant that is non-toxic portion of cholera toxin can be used in process of vaccine design. For this purpose, in the present research, various types of bioinformatics software were used to study physiochemical properties of different refined structures, and validate them using bioinformatics tools as well as allergenic properties. One of the protein sequences with the highest antigenic index was the protein with sequence of Otc, which had a suitable tertiary structure according to the studies performed by the RaptorX server. Codon adaptation index (CAI) of this protein was increased to 0.98. The predicted structure showed stable condition according to thermodynamic analysis of mRNA. Structural analysis of chimeric vaccine-TLR-2 receptor protein–protein docking predicted efficient binding. Methodological approach and results obtained from studying candidate vaccine could be introduced to further experimental validations after efficacy of which was confirmed by experimental immunological assays in order to control and eliminate cholera in the future.