Effect of TNF‑α on the Proliferation and Osteogenesis of Human Periodontal Mesenchymal Stem Cells
Experimental and Therapeutic Medicine(2021)
摘要
The aim of the present study was to investigate the effect of tumor necrosis factor-alpha (TNF-alpha) on the proliferation and osteogenesis of human periodontal mesenchymal stem cells (hPDLSCs). Antigen expression in hPDLSCs was detected by flow cytometry. hPDLSCs were divided into four groups: A control group with no TNF-alpha treatment, and three experimental groups treated with 0.1, 1 and 10 ng/ml TNF-alpha, respectively. The effect of TNF-alpha on proliferation of hPDLSCs in vitro was detected using a Cell Counting Kit-8 assay. Differentiation into an osteogenic lineage was detected by alkaline phosphatase sand alizarin red staining, and the mRNA and protein expression levels of runt-related transcription factor 2 (Runx2), osteocalcin (OCN) and type I collagen (Col-I) were detected using reverse transcription-quantitative PCR and western blot respectively. Following treatment with 10 ng/ml TNF-alpha, proliferation was significantly increased compared with an untreated control group (P<0.01). Additionally, there was a significant inhibition of alkaline phosphatase enzyme activity, alizarin red mineralization node size, and in the gene and protein expression levels of osteogenic differentiation markers, including Runx2, OCN and COL-I (all, P<0.05). Taken together, the results indicated that treatment with 10 ng/ml TNF-alpha promoted the proliferation of hPDLSCs in vitro and inhibited osteogenic differentiation of hPDLSCs, providing an experimental basis for regulation of hPDLSC-mediated periodontal tissue regeneration.
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关键词
tumor necrosis factor-α,human periodontal mesenchymal stem cell,proliferation,osteogenesis,differentiation
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