Micromirror Total Internal Reflection Microscopy for High-Performance Single Particle Tracking at Interfaces.
ACS photonics(2021)
摘要
Single particle tracking has found broad applications in the life and physical sciences, enabling the observation and characterization of nano- and microscopic motion. Fluorescence-based approaches are ideally suited for high-background environments, such as tracking lipids or proteins in or on cells, due to superior background rejection. Scattering-based detection is preferable when localization precision and imaging speed are paramount due to the in principle infinite photon budget. Here, we show that micromirror-based total internal reflection dark field microscopy enables background suppression previously only reported for interferometric scattering microscopy, resulting in nanometer localization precision at 6 μs exposure time for 20 nm gold nanoparticles with a 25 × 25 μm2 field of view. We demonstrate the capabilities of our implementation by characterizing sub-nanometer deterministic flows of 20 nm gold nanoparticles at liquid–liquid interfaces. Our results approach the optimal combination of background suppression, localization precision, and temporal resolution achievable with pure scattering-based imaging and tracking of nanoparticles at interfaces.
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关键词
total internal reflection,dark field microscopy,single particle tracking,light scattering,nanoparticles at fluid interfaces,colloidal flow at fluid interfaces
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