Chrome Extension
WeChat Mini Program
Use on ChatGLM
VIPCreated with Sketch.

SARS病毒S蛋白片段的克隆表达和纯化

Jing CHEN,Yan-yin LI,Yu-sen ZHOU,Yu-ling LI,Chang-lin ZHOU,Hong-qing FANG,Hui-peng CHEN

LETTERS IN BIOTECHNOLOGY（2005）

Cited 0|Views1
No score
Abstract
以大肠杆菌表达载体pET22b为载体,直接表达SARS病毒S蛋白425～569及894～1033等2片段.表达所获得的包涵体形式蛋白经尿素溶解后分别经过2次离子交换层析,获得初步纯化.在酸性和低尿素浓度环境中,2种S蛋白片段极易沉淀.Western印迹鉴定显示其与抗SARS病毒血清呈阳性反应.获得的纯化蛋白可用于检验受体结合能力等研究.
More
Translated text
Key words
protein,purification,sars-cov
AI Read Science
Must-Reading Tree
Example
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined