SARS病毒S蛋白片段的克隆表达和纯化
LETTERS IN BIOTECHNOLOGY(2005)
Abstract
以大肠杆菌表达载体pET22b为载体,直接表达SARS病毒S蛋白425~569及894~1033等2片段.表达所获得的包涵体形式蛋白经尿素溶解后分别经过2次离子交换层析,获得初步纯化.在酸性和低尿素浓度环境中,2种S蛋白片段极易沉淀.Western印迹鉴定显示其与抗SARS病毒血清呈阳性反应.获得的纯化蛋白可用于检验受体结合能力等研究.
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Key words
protein,purification,sars-cov
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