Photoblueing of organic dyes can cause artifacts in super-resolution microscopy
NATURE METHODS(2021)
摘要
Illumination of fluorophores can induce a loss of the ability to fluoresce, known as photobleaching. Interestingly, some fluorophores photoconvert to a blue-shifted fluorescent molecule as an intermediate on the photobleaching pathway, which can complicate multicolor fluorescence imaging, especially under the intense laser irradiation used in super-resolution fluorescence imaging. Here, we discuss the mechanisms of photoblueing of fluorophores and its impact on fluorescence imaging, and show how it can be prevented.
更多查看译文
关键词
Biophysical chemistry,Single-molecule biophysics,Life Sciences,general,Biological Techniques,Biological Microscopy,Biomedical Engineering/Biotechnology,Bioinformatics,Proteomics
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要