Intranasal Delivery of Experimental Compounds in Orthotopic Brain Tumor Mouse Models.

Effective therapeutics for malignant primary brain tumors, such as glioblastomas (GBMs), are urgently needed. To facilitate and expedite early-phase GBM thera-peutic development, we describe a protocol that allows the intranasal delivery of experimental compounds in GBM orthotopic mouse models. Compounds deliv-ered through this route can bypass the blood-brain barrier and thus help validate effective therapeutic targets for GBMs.For complete details on the use and execution of this protocol, please refer to Pinkham et al. (2019).