Microvesicles Secreted by Human Embryonic Stem Cell Derived Mesenchymal Stem Cells Promote Regeneration of Sprague-Dawley Rat Sciatic Nerve

Lei Zhang,Yongbin Ma,Hui Wang,Jianyi Gao,Kai Ye,Tianyan Chen,Jiahong Yu,Bin Tang,Murugan Ramalingam,Jiabo Hu

JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING（2020）

Cited 2|Views21

Abstract

Peripheral nerve injure seriously endangers human health and new methods involving stem cells are currently being developed, these cells may exert their function primarily through secretory microvesicles (MVs) and various small molecules. MVs have become the key mediators of intercellular communication. In this study, MVs secreted by human embryonic stem cell-derived mesenchymal stem cells (hESC-MSC-MVs) were isolated by ultracentrifugation. A Sprague-Dawley rat model of sciatic nerve crush injury was established with 36 Sprague-Dawley rats. Efficacy evaluations including rat footprint test, the HE staining of nerve fibers and the HE staining of gastrocnemius muscle fibers were conducted two weeks after the injection of hESC-MSC-MVs through caudal vein. Results showed that hESC-MSC-MVs obviously had an effect of nerve injury repair and inhibited the denervation atrophy of gastrocnemius muscles. This implied that hESC-MSC-MVs with great regenerative properties might offer an alternative therapeutic option for treating peripheral neuropathy.

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Key words

Human Mesenchymal Stem Cell,Microvesicles,Crush,Sciatic Nerve Injury,Human Embryonic Stem Cell

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Pretraining has recently greatly promoted the development of natural language processing (NLP)
We show that M6 outperforms the baselines in multimodal downstream tasks, and the large M6 with 10 parameters can reach a better performance
We propose a method called M6 that is able to process information of multiple modalities and perform both single-modal and cross-modal understanding and generation
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