Mucilaginibacter Glaciei Sp. Nov. And Mucilaginibacter Pankratovii Sp. Nov., Isolated From A Glacier On The Tibetan Plateau

Two Gram-stain-negative, aerobic, rod-shaped, non-motile, bacterial strains, designated as ZB1P21(T) and ZT4R22(T), were isolated from ice and cryoconite samples collected from Zepu glacier on the Tibetan Plateau, PR China. The phylogenetic analysis of 16S rRNA gene showed that the two strains belong to the genus Mucilaginibacter. Strain ZB1P21(T) showed the highest similarity to Mucilaginibacter rigui WPCB133(T) (97.35 %), while strain ZT4R22(T) showed the highest similarity to Mucilaginibacter gilvus F01003(T) (99.11%). The average nucleotide identity values between the two novel strains and their closest relatives were 79.42 and 85.72% respectively. The two novel strains contained MK-7 as the major menaquinone, and summed feature 3 (comprising C-16:1 omega 7c and/or C-16:1 omega 6c), iso-C-15:0, iso-C-17:0 3-OH and C-16:1 omega 5c as the major fatty acids. The major polar lipid of the two novel strains were phosphatidylethanolamine. Based on these data, we propose two novel species, Mucilaginibacter glaciei sp. nov. (ZB1P21(T)=CGMCC 1.23981(T)=NBRC 113932(T)) and Mucilaginibacter pankratovii sp. nov. (ZT4R22(T)=CGMCC 1.23487(T)=NBRC 113931(T)).