Solimonas Marina Sp. Nov., Isolated From Deep Seawater Of The Pacific Ocean

A taxonomic study was carried out on strain C16B3(T), which was isolated from deep seawater of the Pacific Ocean. The bacterium was Gram-stain-negative, oxidase- and catalase-positive and rod-shaped. Growth was observed at salinities of 0-8.0 % and at temperatures of 10-45 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain C16B3(T) belonged to the genus Solimonas, with the highest sequence similarity to Solimonas terrae KIS83-12(T) (97.2%), followed by Solimonas variicoloris MN28(T) (97.0%) and the other four species of the genus Solimonas (94.5-96.8%). The average nucleotide identity and estimated DNA-DNA hybridization values between strain C16B3(T) and the type strains of the genus Solimonas were 74.05-79.48% and 19.5-22.5 %, respectively. The principal fatty acids (>5 %) were summed feature 8 (C-18:1 omega 7c/C-18:1 omega 6c; 20.9%), iso-C-16:0 (14.6%), C-16:1 omega 5c (9.4 %), iso-C-12:0 (8.4%), summed feature 2 (C-14:0 3-OH/iso I-C-16:1 and C-12:0 aldehyde; 6.8 %) and C-16:0(5.5%). The G+C content of the chromosomal DNA was 65.37 mol%. The respiratory quinone was determined to be Q-8(100%). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unidentified aminolipids, six unidentified phospholipids and one unidentified polar lipid. The combined genotypic and phenotypic data show that strain C16B3(T) represents a novel species within the genus Solimonas, for which the name Solimonas marina sp. nov. is proposed, with the type strain C16B3(T) (=MCCC 1A04678(T)=KCTC 52314(T)).