Antioxidant Effect of Human Selenium-Containing Single-Chain Fv in Rat Cardiac Myocytes

Reactive oxygen species (ROS) plays a key role in human heart diseases. Glutathione peroxidase (GPX) functions as an antioxidant as it catalyzes the reduction of hydroperoxide. In order to investigate the antioxidant effect of human selenium-containing single-chain Fv(Se-scFv-B3), a new mimic of GPX, a model system of hydrogen peroxide (H2O2)-induced rat cardiac myocyte damage was established. The cardiac myocyte damage was characterized in terms of cell viability, lipid peroxidation, cell membrane integrity, and intracellular H2O2 level. The Se-scFv-B3 significantly reduced H2O2-induced cell damage as shown by the increase of cell viability, the decline of malondialdehyde (MDA) production, lactate dehydrogenase (LDH) release, and intracellular H2O2 level. So Se-scFv-B3 may have a great potential in the treatment of human heart diseases induced by ROS.