Comparative Genomic Analysis of Recommended Primer Sets for COVID-19 (Sars-Cov-2) Coronavirus Infection Diagnosis Revealed the Best One

Coronaviruses are RNA viruses that can infect humans and animals. The most famous representatives of the genus Betacoronavirus are SARS-CoV, MERS-CoV and the new 2019-nCoV. Today, the task of detecting a new coronavirus is urgent. We carried out a bioinformatics analysis of the available in the literature and recommended by the WHO and the governments of some country's primer sets for real-time RT-PCR detection. Based on mutational variability of the genome of the closely related SARS-CoV virus, a map of conservative and variable regions was constructed. Two sets of primers were selected that will show themselves in the most efficient way, since they anneal not only to the conservative areas of 2019-nCoV, but also to conservative areas of SARS-CoV genome, therefore, possible mutational variation in the genome of the new coronavirus will not affect the results of the diagnosis of the disease.