Short Report: Persistence of Viral Rna in Chikungunya Virus-Infected Aedes Aegypti (diptera: Culicidae) Mosquitoes after Prolonged Storage at 28 Degrees C
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE(2012)
摘要
Experiments were conducted to determine the persistence of chikungunya viral (CHIKV) RNA in experimentally infected Aedes aegypti mosquitoes stored for prolonged periods at 28 degrees C. Intra-thoracically inoculated mosquitoes with confirmed positivity were killed by quick freezing at -80 degrees C, applied to sticky tape, and stored at 28 degrees C with 80 +/- 5% relative humidity (RH). At weekly intervals, five mosquitoes were removed from the tape randomly and assayed individually for detection of viral RNA by reverse transcriptase-polymerase chain reaction (RT-PCR). CHIKV RNA was detected up to 12 weeks in dry mosquitoes by RT-PCR. Virus could not be isolated either in cell culture or in the suckling Swiss-albino mouse system at any stage. This study demonstrated the persistence of CHIKV viral RNA up to 12 weeks when stored at 28 degrees C with RH 80 +/- 5%. This finding will have significance in CHIKV surveillance programs in mosquito populations or field-based studies in countries where maintenance of a cold chain is a concern.
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