Effect of 1-Allyl-3-methylimidazoleum Bromide on the Motional Dynamics and Thermal Stability of Horse Ferrocytochrome C

The novel green solvent, ionic liquids emerged as potential solvents in executing the various in-vitro protein based applications. In the present study, we investigated the role of 1-allyl-3-methylimidazolium bromide ([Amim]Br) on motional dynamics and thermal stability of ferrocytochrome c (Ferrocyt c) at pH 7.0. Our results revealed that (i) the presence of low concentration of [Amim]Br reduces the levels of thermal motion of the.-loop of the native Ferrocyt c (ii) at relatively higher concentrations, the denaturing effect of [ Amim]Br increases the thermal motions of the.-loop that lead to unfold the proteins, and (iii) [Amim]Br presence in reaction medium decreases the thermal stability of native Ferrocyt c at pH 7.0.