Eriocalyxin B Induces Apoptosis And Inhibits Migration Through Down-Regulation Of Rtkn In Human Colon Cancer

Like many epithelial-derived cancers, colon cancer results from a multistep tumorigenic process. However, the detailed mechanisms involved in colon cancer formations are poorly characterized. This study was aimed to elucidate the antitumor effect of Eriocalyxin B in colon cancer LOVO cells and the possible molecular mechanism involved. The results showed that Eriocalyxin B could inhibit the growth and migration, and induce apoptosis of LOVO cells significantly in a dose-dependent manner. The expression level of RTKN was significantly decreased after LOVO cells treated with Eriocalyxin B. However, these effects of Eriocalyxin B were reversed by overexpression of RTKN in LOVO cells. Furthermore, the expression level of NF-kappa Bp65 was significantly increased in Eriocalyxin B-treated LOVO cells with RTKN overexpression. We therefore conclude that Eriocalyxin B exhibited significant growth and migration inhibition of LOVO cells and induced apoptosis of LOVO cells via the down-regulation of RTKN expression and inhibition of NF-kappa B activation.