Casein hydrolyzate augments antimicrobial and antioxidative persistence of cheddar whey protein concentrate based edible coatings
semanticscholar(2017)
摘要
Hydrolysis of casein using chymotrypsin results in the formation of polypeptides (casein hydrolyzate, CH) with a hydrophobic aromatic amino acid on one end of the chain because the enzyme selectively cleaves the adjacent peptidebond. Due to resonance of the aromatic micro-domain, thiols become redox-sensitive and actively participate in electron transfer. These types of amphipathic peptides also tend to be membrane-lytic. The two prong approach of this investigation was to, (1) assess antibacterial effect of the CH in beef steak, and (2), to determine its antioxidative efficacy as a constituent of Cheddar whey based edible coating mix. Coliform growth, using shoulder-cut beefsteaks as model, showed a significant (P<0.05) five log reduction to log CFU<1 even at its lowest concentration range (0.15-0.2 %) (w/v). Radical quenching ability reflected by increasedn time required for maximal alkoxyland peroxyl-radical accumulation in reaction mixtures without and with CH, by in vitro pyrolysis of 2, 2’-Azo-bis (2-amidinopropane), was six minutes even at its lowest trace concentration (0.1 %, w/v) indicating dramatic enhancement of long-term antioxidative persistence. At the same concentration, CH augmented 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging efficacy of the film by 28%. The study showed the potential for beneficial use of CH in dramatically enhancing efficacy of WPC based edible coatings to reduce microbial and oxidative degradation of muscle foods during retail-cut processing and display.
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