Nete Uptake and Aceumulation ofExogenous JsgA Docosahexaenoic Acid by Chlorezaa

Tuna oil or its hydrolysate was added to a culture of C7ilorella for its nutritional fortification as a feed for rotifer. Exogenous docosahexaenoic acid (DHA) in its free form was taken up by the cells of thtorelta vuigaris strain K-22 and by other strains, but tuna oiJ was not taken up by the cells. Accumulated DHA was found by electron microscopy in the cells in oil droplets. All strains of Chloretla used in these experiments took up exogenous DHA into the cells. It seems that the structure of the cell wall clid not affect the uptake of DHA into the Chlorella cells.