�nti�icro�ial Pressure o� �ipro�o�acin and Genta�icin on �io�l��e�elop�ent �� an �ndoscope�Isolated Pseudomonas aeruginosa

is work aims at characterizing endoscope bio�lm-isolated �PAI� and reference strain P. aeruginosa �PA� adhesion, bio�lm formation and sensitivity to antibiotics. e recovery ability of the bio�lm-growing bacteria sub�ected to intermittent antibiotic pressure �cipro�oxacin �CIP� and gentamicin �GM��, as well as the development of resistance towards antibiotics and benzalkonium chloride �BC�, were also determined.e capacity of both strains to develop bio�lmswas greatly impaired in the presence ofCIP and GM. Sanitization was not complete allowing bio�lm recovery a�er the intermittent cycles of antibiotic pressure.e environmental pressure exerted by CIP and GM did not develop P. aeruginosa resistance to antibiotics nor cross-resistance towards BC. However, data highlighted that none of the antimicrobials led to complete bio�lm eradication, allowing the recovery of the remaining adhered population possibly due to the selection of persister cells. is feature may lead to bio�lm recalcitrance, reinforcement of bacterial attachment, and recolonization of other sites.