Homogeneous No-Wash AlphaLISA Assays for Genetically-Modified ( GMO ) Protein Detection in Seed Extracts

Genetically modified (GM) crops are widely used in the agricultural industry. These crops are manipulated to possess traits which can make them less susceptible to pests, more resistant to herbicides, allow for the production of more nutritionally dense food, and even produce pharmaceutical drugs. As the use of GM crops becomes more prevalent, it is imperative that rapid and sensitive tools exist for the detection of GM proteins from plants. Traditional methods for detection include labor-intensive multi-wash ELISA kits or strip-based tests which are not quantitative. Here, we present two assays using AlphaLISA Technology that allow for the detection and quantitation of transgenic proteins from seed extracts in under two hours, without any wash steps. Homogeneous No-Wash AlphaLISA Assays for Genetically-Modified (GMO) Protein Detection in Seed Extracts A P P L I C A T I O N N O T E