Resolving the benign and the malign isomers of aryl amines by HPLC

Aryl amines are widely used as precursors in the manufacturing of dyes, medicines, pesticides, adhesives, polymers and hence gain significance in their analysis and exact identification[1]. In fact the large usage of aryl amines is for making dyes. Azo dyes are extensively used in industrial application since 19 century, for their colorfastness and being cost-effective. Azo dyes are used for coloring many consumer goods like toys, leather, clothes, cosmetics and plastics[2, 3] and also used in foods[4]. They undergo reduction biologically and release aromatic amines. This reduction is done by the azo reductases present in sweat, intestinal bacteria, skin and liver cells as reported[2, 3, 5-7]. Some of the aroPriyaNarayanan, R.Ganeshjeevan*, S.Suresh, C.Muralidharan Central Instrumentation Lab and Eco Analytical Lab and CSIR-Central Leather Research Institute, Sardar patel road, Adyar, Chennai, T.N., (INDIA) E-mail: clriexcel@yahoo.com