Confirmation Of Large Leaf Gentian (Gentiana Macrophylla Pall.) Identity By Thin-Layer Chromatography

Large-leaf gentian herb is a promising source of biologically active substances, which is actively used in the traditional medicine of many countries. It contains flavonoids, iridoids, secoiridoids, polysaccharides, xanthones with a wide spectrum of pharmacological activity. The aim of the research was to establish a procedure for identification of large-leaved gentian (Gentiana macrophylla Pall.) using thin-layer chromatography (TLC). In order to identify both hydrophilic and hydrophobic compounds in extracts from the plant two mobile phases were proposed which allow distinguishing specific zones by their color and relative position. Validation of developed procedure was carried out in terms of specificity and chromatographic system suitability. The specificity of the procedure was assessed by matching of TLC profiles for all studied samples. It was found that the main zones on the chromatograms are matching. Thus, chromatographic characteristics of large leaf gentian aerial parts were established for the first time using validated TLC procedure. The results of the study can be used for standardization of large-leaved gentian herb.