Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins

We describe two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria and tumors in the mouse body and brain. We developed machine learning-based software that allows commercial imaging systems to be used for temporal unmixed OA imaging, enabling its routine use in life sciences. ### Competing Interest Statement VN is a shareholder of iThera Medical